There is increasing interest in the use of flow cytometry for phenotypic analysis of small particles such as extracellular vesicles and viruses. To detect these small particles, conventional flow cytometers are functioning at the limit of detection. There is currently no consensus on how small particles should be analysed on flow cytometers. This session brings together small particle flow cytometry gurus from around the world to share their different approaches to resolving the small stuff.

Topics of Discussion:

  • Pros and cons of SSC vs FL threshold
  • Calibration methods and and the materials to use (FL & SSC)

Guest Panelists: 

  • Edwin van der Pol (Amsterdam University Medical Centers/Exometry)
  • Desmond Pink (Nanostics)
  • Joanne Lannigan (Flow Cytometry Support Services)
  • John Nolan (Scintillon Institute/Cellarcus)


  • Joshua Welsh (NIH)
  • Vera Tang (University of Ottawa)

Meet the Cores of Canada: 

McGill Flow Cytometry Core Facility

Session Recording:


Series: BD Biosciences


  • Apogee Flow Systems
  • Beckman Coulter Life Sciences
  • Bio-Techne
  • Cytek Biosciences
  • Luminex